• Catalog Number:

  E1A0450A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ku80 around the phosphorylation site of threonine 714 (G-D-TP-A-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P13010

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Alter Name:

  86 kDa subunit of Ku antigen, ATP-dependent DNA helicase II, 80 kDa subunit, CTC box binding factor 85 kDa subunit, CTC85, CTCBF, DNA-repair protein XRCC5, G22P2, KU86, Lupus Ku autoantigen protein p86, Nuclear factor IV, TLAA, Thyroid- lupus autoantigen, XRCC5

• Specificity Sensitivity:

  Ku80 (Phospho-Thr714) antibody detects endogenous levels of Ku80 only when phosphorylated at threonine 714.

• References:

  Catherine L. Bladen, Nucleic Acids Res., May 2005; 33: 3002 - 3010. Dae-Sik Lim, Mol. Cell. Biol., Jun 2000; 20: 3772 - 3780. Joo Weon Lim, J. Biol. Chem., Nov 2002; 277: 46093 - 46100. B. K. Singleton, Mol. Cell. Biol., May 1999; 19: 3267 - 3277..

• All Sites:

  Human: Thr714

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Ku80 (Phospho-Thr714) Antibody (#A0450).

Western blot analysis of extracts from COS7 cells using Ku80 (Phospho-Thr714) Antibody (#A0450).

P-peptide - + Immunofluorescence analysis of HeLa cells, using Ku80 (Phospho-Thr714) Antibody (#A0450).