• Catalog Number:

  E1A0449A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ku70 around the phosphorylation site of serine 5 (W-E-SP-Y-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P12956

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  70 kDa subunit of Ku antigen, ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC75, CTCBF, G22P1, Lupus Ku autoantigen protein p70, TLAA, Thyroid-lupus autoantigen

• Specificity Sensitivity:

  Ku70 (Phospho-Ser5) antibody detects endogenous levels of Ku70 only when phosphorylated at serine 5.

• References:

  Nicholas S. Y. Ting, Nucleic Acids Res., Apr 2005; 33: 2090 - 2098. Suparna Mazumder, Mol. Cell. Biol., May 2007; 27: 3511 - 3520. Roopashree Narasimhaiah, Cereb Cortex, Jun 2005; 15: 696 - 707.

• All Sites:

  Human: Ser5; Mouse: Ser6

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Ku70 (Phospho-Ser5) antibody. Western blot analysis of extracts from HeLa cells, using Ku70 (Phospho-Ser5) antibody.