• Catalog Number:

  E1A0447A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 612 (P-M-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  IRS-1, IRS1, Insulin receptor substrate-1

• Specificity Sensitivity:

  IRS-1 (Phospho-Ser612) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 612.

• References:

  XJ Sun, J. Biol. Chem., Nov 1992; 267: 22662 - 22672. Laurent Delahaye, Endocrinology, Dec 1998; 139: 4911. Angela M. Valverde, Mol. Cell. Biol., Apr 2001; 21: 2269 - 2280. Xiao Jian Sun, J. Biol. Chem., May 1996; 271: 10583. ----------------------------------------------------------------------------

• All Sites:

  Human: Ser616; Mouse: Ser612; Rat: Ser612

Western blot analysis of extracts from HUVEC cells, treated with insulin (0.01U/ml, 30mins), using IRS-1 (Phospho-Ser612) antibody.