• Catalog Number:

  E1A0446A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 1101 (H-S-SP-E-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  IRS-1, IRS1, Insulin receptor substrate-1

• Specificity Sensitivity:

  IRS-1 (Phospho-Ser1101) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 1101.

• References:

  XJ Sun, J. Biol. Chem., Nov 1992; 267: 22662 - 22672. Laurent Delahaye, Endocrinology, Dec 1998; 139: 4911. Angela M. Valverde, Mol. Cell. Biol., Apr 2001; 21: 2269 - 2280. Xiao Jian Sun, J. Biol. Chem., May 1996; 271: 10583.

• All Sites:

  Human: Ser1101; Mouse: Ser1097; Rat: Ser1100

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IRS-1 (Phospho-Ser1101) Antibody (#A0446).

Western blot analysis of extracts from COS7 cells, treated with Calyculin A (50ng/ml, 30mins), using IRS-1 (Phospho-Ser1101) Antibody (#A0446).