• Catalog Number:

  E1A0444A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IL3R around the phosphorylation site of tyrosine 593 (G-P-YP-L-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P26951

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  Interleukin-3 receptor alpha chain, IL-3R-alpha, CD123 antigen, IL3RA

• Specificity Sensitivity:

  IL3R (Phospho-Tyr593) antibody detects endogenous levels of IL3R only when phosphorylated at tyrosine 593.

• References:

  Michelle M. Le Beau, Blood, Aug 2003; 102: 1072 - 1074. Elena Cattaneo, J. Biol. Chem., Sep 1996; 271: 23374. Patricia McLoughlin, J. Biol. Chem., Sep 2002; 277: 37045 - 37053. Louise Edvardsson, Blood, Nov 2004; 104: 4120.

• All Sites:

  Human: Tyr593; Mouse: Tyr595

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using IL3R (Phospho-Tyr593) antibody.

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 15mins), using IL3R (Phospho-Tyr593) antibody.