• Catalog Number:

  E1A0442A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKK-β around the phosphorylation site of tyrosine 188 (L-Q-YP-L-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14920

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  EC 2.7.11.10, I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, IKKB, IkBKB, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta, kinase IKK-beta

• Specificity Sensitivity:

  IKK-β (Phospho-Tyr188) antibody detects endogenous levels of IKK-β only when phosphorylated at tyrosine 188.

• References:

  Kelly M. Regula, J. Biol. Chem., Oct 2002; 277: 38676 - 38682. Wen-Hui Hu, J. Biol. Chem., Aug 2005; 280: 29233 - 29241. Irina V. Budunova, AACR Meeting Abstracts, Mar 2004; 2004: 1056. Fan Yang, J. Immunol., Jun 2003; 170: 5630.

• All Sites:

  Human:Tyr188 ; Mouse: Tyr188 ; Rat: Tyr188

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IKK-β (Phospho-Tyr188) antibody (#A0442).

Western blot analysis of extracts from COS7 cells, using IKK-β (Phospho-Tyr188) antibody (#A0442).