Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IκB-β around the phosphorylation site of threonine 19 (C-D-TP-G-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
Q15653
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
I-kappa-B-beta, IKB-B, IKBB, IkappaBbeta, NF-kappa-BIB, NF-kappaB inhibitor beta, NFKBIB, TR- interacting protein 9, TRIP9, Thyroid receptor interacting protein 9
Specificity Sensitivity:
IκB-β (Phospho-Thr19) antibody detects endogenous levels of IκB-β only when phosphorylated at threonine 19.
References:
M. Tewari, Nucleic Acids Res., Jun 1992; 20: 2931.
Raffaele Marfella, J. Am. Coll. Cardiol., Jun 2006; 47: 2444 - 2455.
Manorama Tewari, Nucleic Acids Res., Feb 1992; 20: 607.
All Sites:
Human: Thr19
Western blot analysis of extracts from 239 cells, treated with TNF (20ng/ml, 30mins), using IκB-β (Phospho-Thr19) antibody.