• Catalog Number:

  E1A0437A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSL around the phosphorylation site of serine 552 (R-R-SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05469

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 3.1.1.79, Hormone sensitive lipase, LIPE, LIPS

• Specificity Sensitivity:

  HSL (Phospho-Ser552) antibody detects endogenous levels of HSL only when phosphorylated at serine 552.

• References:

  Matthew J. Watt, Am J Physiol Endocrinol Metab, Mar 2006; 290: E500 - E508. Kenji Harada, Am J Physiol Endocrinol Metab, Dec 2003; 285: E1182 - E1195. Shu Pei Wang, Endocrinology, Dec 2004; 145: 5688 - 5693. Mélanie Fortier, J. Lipid Res., Sep 2005; 46: 1860 - 1867.

• All Sites:

  Human: Ser552; Mouse: Ser557; Rat: Ser563

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSL (Phospho-Ser552) antibody. Western blot analysis of extracts from K562 cells, using using HSL (Phospho-Ser552) antibody.