• Catalog Number:

  E1A0436A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 259 (T-A-SP-E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q9UQL6

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Specificity Sensitivity:

  HDAC5 (Phospho-Ser259) antibody detects endogenous levels of HDAC5 only when phosphorylated at serine 259.

• References:

  Claudie Lemercier, J. Biol. Chem., May 2000; 275: 15594. changhoon ha, FASEB J, Apr 2007; 21: A528. Jinjiang Pang, FASEB J, Apr 2007; 21: A1151. Carmen C. Sucharov, Am J Physiol Cell Physiol, Nov 2006; 291: C1029 - C1037.

• All Sites:

  Human: Ser259; Mouse: Ser250

Western blot analysis of extracts from HepG2 cells, using HDAC5 (Phospho-Ser259) antibody (#A0436).