• Catalog Number:

  E1A0435A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of serine 28 (R-K-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P68431

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Alter Name:

  H3/a, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, histone H3.1

• Specificity Sensitivity:

  Histone H3 (Phospho-Ser28) antibody detects endogenous levels of Histone H3 only when phosphorylated at serine 28.

• References:

  N Chodchoy, Mol. Cell. Biol., Mar 1987; 7: 1039 - 1047. Tomoko HAYASHI, J. Biochem. (Tokyo), Jun 1984; 95: 1741 - 1749. Scott C. Galasinski, J. Biol. Chem., Jan 2002; 277: 2579 - 2588. Sandra B. Hake, J. Biol. Chem., Jan 2006; 281: 559 - 568.

• All Sites:

  Human: Ser28; Mouse: Ser28; Rat: Ser29

Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho-Ser28) antibody (#A0435).