• Catalog Number:

  E1A0433A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GRF-1 around the phosphorylation site of tyrosine 1105 (N-I-YP-S-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9NRY4

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  GAP-associated protein p190, GRF1, GRLF, GRLF1, Glucocorticoid receptor DNA binding factor 1, Glucocorticoid receptor repression factor 1, KIAA1722, Rho GAP p190A, p190-A

• Specificity Sensitivity:

  GRF-1 (Phospho-Tyr1105) antibody detects endogenous levels of GRF-1 only when phosphorylated at tyrosine 1105.

• References:

  Tikoo A., Gene 257:23-31(2000). Nagase T., DNA Res. 7:347-355(2000). Nakajima D., DNA Res. 9:99-106(2002).

• All Sites:

  Human: Tyr1105; Mouse: Tyr943; Rat: Tyr1105

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using GRF-1 (Phospho-Tyr1105) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using GRF-1 (Phospho-Tyr1105) antibody.