• Catalog Number:

  E1A0432A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GR around the phosphorylation site of serine 226 (L-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04150

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  GCR, GRL, GRL1, NR3C1, glucocorticoid receptor

• Specificity Sensitivity:

  GR (Phospho-Ser226) antibody detects endogenous levels of GR only when phosphorylated at serine 226.

• References:

  Kyung Jin Kwak, J. Exp. Bot., Nov 2005; 56: 3007 - 3016. Maite de Llanos, Hum. Reprod., Jan 2005; 20: 216 - 220. M. Lynch, Mol. Hum. Reprod., Jul 2005; 11: 507 - 512. Valeriya P. Makarenkova, J. Immunol., Feb 2006; 176: 2085 - 2094.

• All Sites:

  Human: Ser226; Mouse: Ser234; Rat: Ser246

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using GR (Phospho-Ser226) antibody (#A0432).

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Phospho-Ser226) antibody (#A0432).