• Catalog Number:

  E1A0431A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Glycogen Synthase around the phosphorylation site of serine 645 (P-P-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P13807

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  EC 2.4.1.11, GYS, glycogen synthase muscle

• Specificity Sensitivity:

  Glycogen Synthase (Phospho-Ser645) antibody detects endogenous levels of Glycogen Synthase only when phosphorylated at serine 645.

• References:

  Didier Laurent, Am J Physiol Endocrinol Metab, Apr 2000; 278: E663 - E668. Young Soo Ahn, Carcinogenesis, Dec 1992; 13: 2329 - 2334. Alina Vos, J. Biol. Chem., Apr 2007; 10.1074. Jörg Hans Hacker, Carcinogenesis, Dec 1990; 11: 2199 - 2204.

• All Sites:

  Human: Ser645; Mouse: Ser645; Rat: Ser644

P-peptide - + Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using Glycogen Synthase (Phospho-Ser645) antibody (#A0431).

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using Glycogen Synthase (Phospho-Ser645) antibody (#A0431).