• Catalog Number:

  E1A0430A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-YP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P06241

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  EC 2.7.10.2, FYN, P59-FYN, SLK, SYN, kinase Fyn

• Specificity Sensitivity:

  Fyn (Phospho-Tyr530) antibody detects endogenous levels of Fyn only when phosphorylated at tyrosine 530.

• References:

  Weijie Li, J. Biol. Chem., Jan 2007; 282: 1161 - 1169. Xiaowu Li, J. Biol. Chem., Oct 2003; 278: 41646 - 41653. Mamiko Maekawa, Biol Reprod, Jan 2002; 66: 211. Jeannie Chin, J. Neurosci., Oct 2005; 25: 9694 - 9703.

• All Sites:

  Human: Tyr530; Mouse: Tyr527

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Fyn (Phospho-Tyr530) antibody (#A0430).

Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Fyn (Phospho-Tyr530) antibody (#A0430).