• Catalog Number:

  E1A0429A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Fos around the phosphorylation site of serine 362 (K-G-SP-S-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P01100

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos

• Specificity Sensitivity:

  Fos (Phospho-Ser362) antibody detects endogenous levels of Fos only when phosphorylated at serine 362.

• References:

  Hai T., Genes Dev. 4:682-682(1990). Heilig R., Nature 421:601-607(2003). Vasilescu J., J. Proteome Res. 6:298-305(2007).

• All Sites:

  Human: Ser362; Mouse: Ser362; Rat: Ser362

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using Fos (Phospho-Ser362) antibody. Western blot analysis of extracts from K562 cells, treated with forskolin (40nM, 30mins), using Fos (Phospho-Ser362) antibody.