• Catalog Number:

  E1A0427A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FANCD2 around the phosphorylation site of serine 222 (G-D-SP-Q-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q9BXW9

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  FACD2, FANCD2, Fanconi anemia complementation group D2 protein

• Specificity Sensitivity:

  FANCD2 (Phospho-Ser222) antibody detects endogenous levels of FANCD2 only when phosphorylated at serine 222.

• References:

  Shobbir Hussain, Hum. Mol. Genet., Jun 2004; 13: 1241 - 1248. Rocio Montes de Oca, Blood, Feb 2005; 105: 1003 - 1009. XiaoZhe Wang, Mol. Cell. Biol., Jul 2004; 24: 5850 - 5862. Gary P. H. Ho, Mol. Cell. Biol., Sep 2006; 26: 7005 - 7015.

• All Sites:

  Human: Ser222

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FANCD2 (Phospho-Ser222) antibody (#A0427).

Western blot analysis of extracts from HT-29 cells, treated with Calyculin A (50ng/ml, 30mins), using FANCD2 (Phospho-Ser222) antibody (#A0427).