• Catalog Number:

  E1A0426A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 576 (S-T-YP-Y-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  EC 2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2, pp125FAK

• Specificity Sensitivity:

  FAK (Phospho-Tyr576) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 576.

• References:

  Fumin Chang, Mol. Biol. Cell, Jan 2007; 18: 253 - 264. Pamela J. Bruce-Staskal, J. Cell Sci., Jul 2002; 115: 2689. James D. Owen, Mol. Cell. Biol., Jul 1999; 19: 4806 - 4818. Roseanne S. Greenberg, FASEB J, May 2006; 20: 1006 - 1008.

• All Sites:

  Human: Tyr576; Mouse: Tyr614; Rat: Tyr576

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using FAK (Phospho-Tyr576) Antibody (#A0426).

Western blot analysis of extracts from NIH/3T3 cells, using FAK (Phospho-Tyr576) Antibody (#A0426).