• Catalog Number:

  E1A0425A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FADD around the phosphorylation site of serine 194 (A-M-SP-P-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q13158

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  FAS-associating death domain-containing protein, MORT1, Mediator of receptor induced toxicity

• Specificity Sensitivity:

  FADD (Phospho-Ser194) antibody detects endogenous levels of FADD only when phosphorylated at serine 194.

• References:

  Wen-Chen Yeh, Science, Mar 1998; 279: 1954. Carsten Scaffidi, J. Immunol., Feb 2000; 164: 1236. Anna A. Kuang, J. Biol. Chem., Aug 2000; 275: 25065. Guoan Chen, PNAS, Aug 2005; 102: 12507 - 12512.

• All Sites:

  Human: Ser194

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FADD (Phospho-Ser194) Antibody (#A0425).

Western blot analysis of extracts from HeLa cells, treated with Paclitaxel (1μM, 60mins), using FADD (Phospho-Ser194) Antibody (#A0425).