• Catalog Number:

  E1A0423A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human E2F1 around the phosphorylation site of threonine 433 (D-L-TP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q01094

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Specificity Sensitivity:

  E2F1 (Phospho-Thr433) antibody detects endogenous levels of E2F1 only when phosphorylated at threonine 433.

• References:

  Jing W. Zhu, Mol. Cell. Biol., Dec 2001; 21: 8547 - 8564. Ludger Hauck, Mol. Cell. Biol., Apr 2002; 22: 2147 - 2158. Kang Liu, Mol. Cell. Biol., May 2003; 23: 3287 - 3304. J K Hsieh, Genes & Dev., Jul 1997; 11: 1840 - 1852.

• All Sites:

  Human: Thr433; Mouse: Thr426

Western blot analysis of extracts from HeLa cells, treated with Etoposide (25μM, 24hours), using E2F1 (Phospho-Thr433) Antibody (#A0423).