• Catalog Number:

  E1A0418A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin D3 around the phosphorylation site of threonine 283 (T-S-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P30281

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  CCND3, CGD3, Cyl-3, G1/S-specific cyclin D3

• Specificity Sensitivity:

  Cyclin D3 (Phospho-Thr283) antibody detects endogenous levels of Cyclin D3 only when phosphorylated at threonine 283.

• References:

  Guo-Li Wang, Mol. Cell. Biol., Apr 2006; 26: 2570 - 2582. Feng Dong, Mol. Biol. Cell, Aug 1998; 9: 2081. Tapan Kumar Bagui, Mol. Cell. Biol., Dec 2000; 20: 8748 - 8757. David A. Sarruf, Mol. Cell. Biol., Nov 2005; 25: 9985 - 9995.

• All Sites:

  Human: Thr283; Mouse: Thr283; Rat: Thr284

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin D3 (Phospho-Thr283) Antibody (#A0418).

Western blot analysis of extracts from K562 cells treated with UV (5mins), using Cyclin D3 (Phospho-Thr283) Antibody (#A0418).