• Catalog Number:

  E1A0417A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin D1 around the phosphorylation site of threonine 286 (A-C-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P24385

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  BCL-1 oncogene, BCL1, CCND1, CYL-1, G1/S-specific cyclin D1, PRAD1, PRAD1 oncogene

• Specificity Sensitivity:

  Cyclin D1 (Phospho-Thr286) antibody detects endogenous levels of Cyclin D1 only when phosphorylated at threonine 286.

• References:

  Zhiping Li, Mol. Cell. Biol., Jun 2006; 26: 4240 - 4256. Toshiyuki Sakamaki, Mol. Cell. Biol., Jul 2006; 26: 5449 - 5469. Yuki Ishii, J Natl Cancer Inst, Sep 2006; 98: 1238 - 1247.

• All Sites:

  Human: Thr286; Mouse: Thr286

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using Cyclin D1 (Phospho-Thr286) antibody.