• Catalog Number:

  E1A0414A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 387 (C-G-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  CHEK2, CHK2, Cds1, Chk2, RAD53, kinase Chk2

• Specificity Sensitivity:

  Chk2 (Phospho-Thr387) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 387.

• References:

  Atsushi Hirao, Mol. Cell. Biol., Sep 2002; 22: 6521 - 6532. Julie K. Schwarz, Mol. Cancer Res., Jun 2003; 1: 598. Shutong Yang, J. Biol. Chem., Sep 2006; 281: 26645 - 26654. Xingzhi Xu, Mol. Cell. Biol., Jun 2002; 22: 4419 - 4432.

• All Sites:

  Human: Thr387; Mouse: Thr391; Rat: Thr390

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk2 (Phospho-Thr387) Antibody (#A0414).

Western blot analysis of extracts from Jurkat cells, using Chk2 (Phospho-Thr387) Antibody (#A0414).