• Catalog Number:

  E1A0413A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC6 around the phosphorylation site of serine 54 (P-L-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q99741

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  CDC18, CDC6 cell division cycle 6 homolog, CDC6-related protein

• Specificity Sensitivity:

  CDC6 (Phospho-Ser54) antibody detects endogenous levels of CDC6 only when phosphorylated at serine 54.

• References:

  Jonathan R. Hall, Mol. Biol. Cell, Jun 2007; 10.1091. Luo Feng, Mol. Biol. Cell, May 2000; 11: 1673. Arturo Calzada, J. Biol. Chem., Mar 2000; 275: 9734. Rajesh Kasiviswanathan, Nucleic Acids Res., Sep 2005; 33: 4940 - 4950.

• All Sites:

  Human: Ser54; Mouse: Ser55

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDC6 (Phospho-Ser54) antibody (#A0413).

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using CDC6 (Phospho-Ser54) antibody (#A0413).