• Catalog Number:

  E1A0412A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD19 around the phosphorylation site of tyrosine 531 (D-S-YP-E-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P15391

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  B-lymphocyte surface antigen B4, CD19, Leu-12

• Specificity Sensitivity:

  CD19 (Phospho-Tyr531) antibody detects endogenous levels of CD19 only when phosphorylated at tyrosine 531.

• References:

  Manabu Fujimoto, J. Biol. Chem., Nov 2001; 276: 44820 - 44827. LE Bradbury, J. Immunol., Sep 1993; 151: 2915. Minoru Hasegawa, J. Immunol., Sep 2001; 167: 2469.

• All Sites:

  Human: Tyr531; Mouse: Tyr522

Western blot analysis of extracts from COS7 cells, treated with Serum (10%, 30mins), using CD19 (Phospho-Tyr531) Antibody.