• Catalog Number:

  E1A0411A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF1 around the phosphorylation site of serine 63 (R-P-SP-Y-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P18846

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  Activating transcription factor 1; ATF1; Cyclic-AMP-dependent transcription factor ATF-1; TCR-ATF1; TREB36 protein

• Specificity Sensitivity:

  ATF1 (Phospho-Ser63) antibody detects endogenous levels of ATF1 only when phosphorylated at serine 63.

• References:

  Yoshimura T., EMBO J. 9:2537-2542(1990). Rehfuss R.P., J. Biol. Chem. 266:18431-18434(1991). Hai T., Genes Dev. 3:2083-2090(1989).

• All Sites:

  Human: Ser63; Mouse: Ser63

Western blot analysis of extracts from HT-29 cells, treated with Insulin (0.01U/ML, 15mins), using ATF1 (Phospho-Ser63) antibody.