• Catalog Number:

  E1A0410A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPK β1 around the phosphorylation site of serine 181 (S-S-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9Y478

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Alter Name:

  5'-AMP-activated protein kinase beta-1 subunit, AAKB1, AMPK beta-1 chain, AMPK-beta1, PRKAB1, kinase AMPK-beta1

• Specificity Sensitivity:

  AMPK β1 (Phospho-Ser181) antibody detects endogenous levels of AMPK β1 only when phosphorylated at serine 181.

• References:

  Jun Li, Carcinogenesis, May 2003; 24: 827 - 834. Carola U. Niesler, Exp Physiol, Jan 2007; 92: 207 - 217. Angela Woods, J. Biol. Chem., Aug 2003; 278: 28434 - 28442. Zhaohui Feng, Cancer Res., Apr 2007; 67: 3043 - 3053.

• All Sites:

  Human: Ser181; Mouse: Ser181; Rat: Ser181

P-peptide - + Immunohistochemical analysis of paraffin-embedded human skeletal muscletissue using AMPK β1 (Phospho-Ser181) Antibody (#A0410).

Western blot analysis of extracts from Jurkat cells, using AMPK β1 (Phospho-Ser181) Antibody (#A0410).