• Catalog Number:

  E1A0409A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AML-1/RUNX1 around the phosphorylation site of serine 303 (P-I-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q01196

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  Acute myeloid leukemia 1 protein, CBF-alpha 2, CBFA2, Core-binding factor, alpha 2 subunit, Oncogene AML-1, PEA2-alpha B, PEBP2-alpha B, Polyomavirus enhancer binding protein 2 alpha B subunit, RUN1, RUNX1, Runt-related transcription factor 1, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit

• Specificity Sensitivity:

  AML1 (Phospho-Ser303) antibody detects endogenous levels of AML-1/RUNX1 only when phosphorylated at serine 303.

• References:

  S Meyers, Mol. Cell. Biol., Oct 1993; 13: 6336 - 6345. Chaitali Banerjee, PNAS, May 1996; 93: 4968. Jennifer J. Westendorf, Mol. Cell. Biol., Jan 1998; 18: 322 - 333. Ari Melnick, Blood, Dec 2000; 96: 3939 - 3947.

• All Sites:

  Human:Ser303; Mouse: Ser276

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AML1 (Phospho-Ser303) Antibody (#A0409).

Western blot analysis of extracts from Jurkat cells, using AML1 (Phospho-Ser303) Antibody (#A0409).