• Catalog Number:

  E1A0407A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 124 (S-G-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  AKT, AKT1 kinase, C-AKT, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Phospho-Ser124) antibody detects endogenous levels of Akt only when phosphorylated at serine 124.

• References:

  Payaningal R. Somanath, J. Biol. Chem., Jun 2007; 10.1074. Xiaoming Ju, PNAS, May 2007; 104: 7438 - 7443. Brian DeBosch, Circulation, May 2 006; 113: 2097 - 2104.

• All Sites:

  Human: Ser124; Mouse: Ser124; Rat: Ser124

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser124) antibody. Western blot analysis of extracts from mouse brain cells, using Akt (Phospho-Ser124) antibody.