Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Abl around the phosphorylation site of tyrosine 204 (K-L-YP-V-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abl (Phospho-Tyr204) antibody detects endogenous levels of Abl only when phosphorylated at tyrosine 204.
References:
Thomas Burmeister, Blood, Nov 2006; 108: 3556 - 3559.
Kim De Keersmaecker, Blood, Jun 2005; 105: 4849 - 4852.
Florian Klein, J. Exp. Med., Mar 2004; 199: 673.
All Sites:
Human: Tyr204; Mouse: Tyr204; Rat: Tyr204
Western blot analysis of extracts from COS7 cells, Adriamycin (0.5μg/ml, 24 hours), using Abl (Phospho-Tyr204) antibody.