• Catalog Number:

  E1A0401A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 64 (R-N-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13541

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Alter Name:

  4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I

• Specificity Sensitivity:

  4E-BP1 (Phospho-Ser64) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at serine 64.

• References:

  Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422. Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816. Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951. Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.

• All Sites:

  Human: Ser64; Mouse: Ser63; Rat: Ser63

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Ser64) antibody (#A0401).

Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho-Ser64) antibody (#A0401).