• Catalog Number:

  E1A0093A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9H4A3

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  WNK lysine deficient protein kinase 1, KDP, PRKWNK1 PROTEIN KINASE LYSINE-DEFICIENT 1

• Specificity Sensitivity:

  WNK1 (Phospho-Thr58) antibody detects endogenous levels of WNK1 only when phosphorylated at threonine 58.

• References:

  Diana Pacheco-Alvarez, J. Biol. Chem., Sep 2006; 281: 28755 - 28763. Henrik Dimke, Am J Physiol Renal Physiol, Feb 2007; 292: F723 - F735.

• All Sites:

  Human: Thr60; Mouse: Thr58; Rat: Thr58

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using WNK1 (Phospho-Thr58) antibody (#A0093).

Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 30mins), using WNK1 (Phospho-Thr58) antibody (#A0093).