• Catalog Number:

  E1A0092A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SYK around the phosphorylation site of tyrosine 525 (E-N-YP-Y-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43405

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:20000

• Alter Name:

  Spleen tyrosine kinase, PROTEIN-TYROSINE KINASE SYK

• Specificity Sensitivity:

  SYK (Phospho-Tyr525) antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 525.

• References:

  Ludivine Leseux, Blood, Dec 2006; 108: 4156 - 4162. Carol A. Dangelmaier, Blood, May 2005; 105: 3918 - 3924. Carol A. Dangelmaier, Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 627. Clément Couture, J. Biol. Chem., Sep 1996; 271: 24294.

• All Sites:

  Human: Tyr525; Mouse: Tyr519; Rat: Tyr519

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SYK (Phospho-Tyr525) antibody (#A0092).

Western blot analysis of extracts from A549 cells, using SYK (Phospho-Tyr525) antibody (#A0092).