• Catalog Number:

  E1A0091A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SYK around the phosphorylation site of tyrosine 348 (E-V-YP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43405

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  Spleen tyrosine kinase, PROTEIN-TYROSINE KINASE SYK

• Specificity Sensitivity:

  SYK (phospho-Tyr348) antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 348.

• References:

  Jurgen Schymeinsky, Blood, Dec 2006; 108: 3919 - 3927. Clément Couture, J. Biol. Chem., Sep 1996; 271: 24294. Kiyonao Sada, J. Biochem. (Tokyo), Aug 2001; 130: 177 - 186. Hiroshi Harayama, J Androl, Nov 2005; 26: 732 - 740. Vincenzo Di Bartolo, J. Biol. Chem., Mar 1999; 274: 6285.

• All Sites:

  Human: Tyr348; Mouse: Tyr342; Rat: Tyr342

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SYK (phospho-Tyr348) antibody.

Western blot analysis of extracts from COS7, treated with EGF (200ng/ml, 30mins), using SYK (phospho-Tyr348) antibody.