• Catalog Number:

  E1A0090A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT5B around the phosphorylation site of serine 731 (A-P-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P51692

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  Signal transducer and activator of transcription 5B

• Specificity Sensitivity:

  STAT5B (phospho-Ser731) antibody detects endogenous levels of STAT5B only when phosphorylated at serine 731.

• References:

  Zsuzsanna S. Nagy, J. Leukoc. Biol., Oct 2002; 72: 819. Stanislaw M. Stepkowski, Blood, Jan 2002; 99: 680 - 689. Salvador Aznar Benitah, Mol. Biol. Cell, Jan 2003; 14: 40.

• All Sites:

  Human: Ser731; Mouse: Ser730; Rat: Ser730

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5B (phospho-Ser731) antibody.

Western blot analysis of extracts from RAW264.7 cells, treated with EGF (200ng/ml, 30mins), using STAT5B (phospho-Ser731) antibody.