• Catalog Number:

  E1A0088A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SRF around the phosphorylation site of serine 99 (S-L-SP-E-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P11831

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  C-FOS SERUM RESPONSE ELEMENT-BINDING FACTOR

• Specificity Sensitivity:

  SRF (Phospho-Ser99) antibody detects endogenous levels of SRF only when phosphorylated at serine 99.

• References:

  SH Liu, J. Biol. Chem., Oct 1993; 268: 21147 - 21154. Aurore L’honore, Mol. Biol. Cell, Mar 2007; 10.1091/mbc.E06-09-0867. Olaf Heidenreich, J. Biol. Chem., May 1999; 274: 14434. Isabella Irrcher and David A. Hood, J Appl Physiol, Dec 2004; 97: 2207 - 2213.

• All Sites:

  Human: Ser103; Mouse: Ser99; Rat: Ser99

P-peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SRF (Phospho-Ser99) antibody (#A0088).

Western blot analysis of extracts from LOVO cells treated with serum (20%, 15mins), using SRF (Phospho-Ser99) antibody (#A0088).