• Catalog Number:

  E1A0087A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SGK around the phosphorylation site of serine 422 (G-F-SP-Y-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00141

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  SGK1, SERUM/GLUCOCORTICOID-REGULATED KINASE

• Specificity Sensitivity:

  SGK (phospho-Ser422) antibody detects endogenous levels of SGK only when phosphorylated at serine 422.

• References:

  Nicola Perrotti, J. Biol. Chem., Mar 2001; 276: 9406 - 9412. Yun L. Ma, Learn. Mem., Mar 2006; 13: 114 - 118. Ge-Xin Wang, Am J Physiol Heart Circ Physiol, Aug 2004; 287: H533 - H544. Bao-Hong Zhang, J. Biol. Chem., Aug 2001; 276: 31620 - 31626.

• All Sites:

  Human: Ser422; Mouse: Ser422; Rat: Ser421

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SGK (phospho-Ser422) antibody (#A0087).

Western blot analysis of extracts from HeLa cells treated with Insulin (0.01U/ml, 15mins), using SGK (phospho-Ser422) antibody (#A0087, Line 1 and 2).