• Catalog Number:

  E1A0086A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SEPARASE around the phosphorylation site of serine 801 (N-S-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14674

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  Caspase-like protein ESPL1, EC 3.4.22.49, ESP1, ESPL1, Extra spindle poles-like 1 protein, KIAA0165, Separin

• Specificity Sensitivity:

  SEPARASE (Phospho-Ser801) antibody detects endogenous levels of SEPARASE only when phosphorylated at serine 801.

• References:

  Waizenegger I., Curr. Biol. 12:1368-1378(2002). Nagase T., DNA Res. 3:17-24(1996). Zou H., Science 285:418-422(1999).

• All Sites:

  Human: Ser801; Mouse: Ser1189

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.

P-peptide - + Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.