• Catalog Number:

  E1A0085A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rad17 around the phosphorylation site of serine 645 (P-A-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O75943

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  Cell cycle checkpoint protein, DKFZP434A1135, R24L

• Specificity Sensitivity:

  Rad17 (Phospho-Ser645) antibody detects endogenous levels of Rad17 only when phosphorylated at serine 645.

• References:

  Sean M. Post, Nucleic Acids Res., Oct 2003; 31: 5568 - 5575. Lee Zou, Genes & Dev., Jan 2002; 16: 198. Renu Garg, Mol. Cancer Res., Jun 2004; 2: 362 - 369.

• All Sites:

  Human: Ser645; Mouse: Ser656

Western blot analysis of extracts from HeLa cells, treated with UV (15mins), using Rad17 (Phospho-Ser645) antibody.