• Catalog Number:

  E1A0084A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 580 (D-Y-YP-K-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q14289

• Applications:

  WB: 1: 500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  PTK2B protein tyrosine kinase 2 beta isoform a, PTK2B protein tyrosine kinase 2 beta isoform b, calcium-dependent tyrosine kinase, cell adhesion kinase beta, focal adhesion kinase 2, proline-rich tyrosine kinase 2 protein kinase B, related adhesion focal tyrosine kinase

• Specificity Sensitivity:

  PYK2 (phospho-Tyr580) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 580.

• References:

  Yoshiko Banno, J. Biol. Chem., Apr 2005; 280: 16319 - 16324. Robin R. Hodges, Invest. Ophthalmol. Vis. Sci., Aug 2006; 47: 3352 - 3359. Sarah J. McLeod, J. Biol. Chem., Mar 2004; 279: 12009 - 12019. Chong-Shan Shi, J. Biol. Chem., Aug 2000; 275: 24470

• All Sites:

  Human: Tyr580; Mouse: Tyr580; Rat: Tyr580

P-peptide - + Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (phospho-Tyr580) antibody.

Western blot analysis of extracts from K562 cells, using PYK2 (phospho-Tyr580) antibody.