Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 881 (L-V-YP-L-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q14289
Applications:
WB: 1:500~1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
CADTK, CAKB, FADK2, FAK2, PKB, PYK2, RAFTK, CAK beta, PTK2B protein tyrosine kinase 2 beta isoform a, PTK2B protein tyrosine kinase 2 beta isoform b, calcium-dependent tyrosine kinase, cell adhesion kinase beta, focal adhesion kinase 2, proline-rich tyrosine kinase 2 protein kinase B, related adhesion focal tyrosine kinase
Specificity Sensitivity:
PYK2 (Phospho-Tyr881) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 881.
References:
Yoshiko Banno, J. Biol. Chem., Apr 2005; 280: 16319 - 16324.
Andrew D. Burdick, Carcinogenesis, Nov 2006; 27: 2331 - 2340.
Robin R. Hodges, Invest. Ophthalmol. Vis. Sci., Aug 2006; 47: 3352 - 3359.
Jaime Melendez, J. Biol. Chem., Dec 2004; 279: 53516 - 53523.
All Sites:
Human: Tyr881; Mouse: Tyr881; Rat: Tyr881
P-peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr881) antibody (#A0083).
Western blot analysis of extracts from mouse brain, using PYK2 (Phospho-Tyr881) antibody (#A0083, Line 1 and 2).