• Catalog Number:

  E1A0082A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P24928

• Applications:

  WB: 1: 500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Alter Name:

  RPO2; RPOL2,POLR2 ALPHA-AMANITIN RESISTANCE,POLYMERASE II, RNA, SUBUNIT A,RNA POLYMERASE II, 220-KD SUBUNIT,RNA POLYMERASE II, LARGE SUBUNIT,RPB1, S. CEREVISIAE, HOMOLOG OF polymerase (RNA) II (DNA directed) polypeptide A

• Specificity Sensitivity:

  POLR2A (phospho-Ser1619) antibody detects endogenous levels of POLR2A only when phosphorylated at serine 1619.

• References:

  Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600 - 7612. Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833 - 843. Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161.

• All Sites:

  Human: Ser1619; Mouse: Ser1619; Rat: Ser1598

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (phospho-Ser1619) antibody.

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody.

PMA + - Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (phospho-Ser1619) antibody.