• Catalog Number:

  E1A0031A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (phospho-Ser425) antibody detects endogenous levels of Smad3 only when phosphorylated at serine 425.

• References:

  Suvi-Katri Leivonen, AACR Meeting Abstracts, Mar 2004; 2004: 530. Baljit Walia, FASEB J, Sep 2003; 10.1096/fj.02-1211fje. Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552. HUGO CEULEMANS, Physiol Rev, Jan 2004; 84: 1.

• All Sites:

  Human: Ser425; Mouse: Ser425; Rat: Ser425

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (phospho-Ser425) antibody.

Western blot analysis of extracts from NIH/3T3 cells treated with IGF-I (100ng/ml, 10mins), using Smad3 (phospho-Ser425) antibody.