• Catalog Number:

  E1A8546A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TUBA1/3/4 around the phosphorylation site of tyrosine 272 (A-T-YP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Alpha-tubulin 1; TBA1; TBA1A; TBA4A; Testis-specific alpha- tubulin; TUBA1; Tubulin alpha-1 chain; Tubulin H2-alpha

• Specificity Sensitivity:

  TUBA1/3/4 (Phospho-Tyr272) antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at tyrosine 272.

• References:

  Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001). Sugano S., Nat. Genet. 36:40-45(2004). Venter J.C., Submitted (JUL-2005).

• All Sites:

  Human: Tyr272

Western blot analysis of extracts from Rat brain cells, using TUBA1/3/4 (Phospho-Tyr272) antibody.