• Catalog Number:

  E1A8521A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 516/199 (Y-S-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 90%, Positives = 100%); Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P10636

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  MAPT; Microtubule-associated protein tau; MTBT1; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

• Specificity Sensitivity:

  Tau (Phospho-Ser516/199) antibody detects endogenous levels of Tau only when phosphorylated at serine 516/199.

• References:

  Goedert M., Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055(1988). Goedert M., EMBO J. 8:393-399(1989). Lee G., Neuron 2:1615-1624(1989).

• All Sites:

  Human: Ser516; Mouse: Ser1491; Rat: Ser510

Western blot analysis of extracts from Mouse brain cells, using Tau (Phospho-Ser516/199) antibody.