• Catalog Number:

  E1A8520A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAPK1 around the phosphorylation site of tyrosine 204.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  P28482/P27361

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  ERK; ERK-2; ERK2; ERT1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; MAPK1; MAPK2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2

• Specificity Sensitivity:

  MAPK1/3 (Phospho-Tyr205/222) antibody detects endogenous levels of MAPK1 only when phosphorylated at tyrosine 204.

• References:

  Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992). Dunham I., Nature 402:489-495(1999). Dunham I., Nature 402:489-495(1999).

• All Sites:

  Human: Tyr205/222; Mouse: Tyr203/223; Rat: Tyr203/223

Western blot analysis of extracts from JK cells, using MAPK1/3 (Phospho-Tyr205/222) antibody.