• Catalog Number:

  E1A8503A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FADD around the phosphorylation site of serine 191 (N-M-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13158

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  FAS-associating death domain-containing protein; Mediator of receptor induced toxicity; MORT1

• Specificity Sensitivity:

  FADD (Phospho-Ser191) antibody detects endogenous levels of FADD only when phosphorylated at serine 191.

• References:

  Sugano S., Nat. Genet. 36:40-45(2004). Farmer A., Submitted (MAY-2003). Venter J.C., Submitted (JUL-2005).

• All Sites:

  Human: Ser191

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser191) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using FADD (Phospho-Ser191) antibody.