• Catalog Number:

  E1A8483A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 308 (M-K-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%) Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha

• Specificity Sensitivity:

  AKT1 (Phospho-Thr308) antibody detects endogenous levels of AKT1 only when phosphorylated at threon 308.

• References:

  Permutt M.A., Diabetologia 44:910-913(2001). Noguchi M., Mol. Cell 6:395-407(2000). Lienhard G.E., J. Biol. Chem. 277:22115-22118(2002).

• All Sites:

  Human: Thr308

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using AKT1 Phospho-Thr308) antibody.