• Catalog Number:

  E1A8468A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p63 around the phosphorylation site of serine 395 (R-R-SP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9H3D4

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EEC3; KET; LMS; p51; p73H; p73L; SHFM4; TA p63 alpha; TP63; tumor protein p63

• Specificity Sensitivity:

  p63 (Phospho-Ser395) antibody detects endogenous levels of p63 only when phosphorylated at serine 395.

• References:

  Senoo M., Biochem. Biophys. Res. Commun. 248:603-607(1998). Augustin M., Mamm. Genome 9:899-902(1998). Yang A., Mol. Cell 2:305-316(1998).

• All Sites:

  Human: Ser395; Mouse: Ser395; Rat: Ser395

Western blot analysis of extracts from HepG2 cells, A549 cells and LOVO cells all treated with nocodazole (1ug/ml, 18hours), using p63 (Phospho-Ser395) antibody.