• Catalog Number:

  E1A8464A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Lamin A around the phosphorylation site of serine 22 (P-L-SP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatographyusing non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P02545

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  70 kDa lamin; LAMA; LMN1; LMNA

• Specificity Sensitivity:

  Lamin A (Phospho-Ser22) antibody detects endogenous levels of Lamin A only when phosphorylated at serine 22.

• References:

  McKeon F.D., Nature 319:463-468(1986). Fisher D.Z., Proc. Natl. Acad. Sci. U.S.A. 83:6450-6454(1986). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser22; Mouse: Ser22; Rat: Ser22

Western blot analysis of extracts from HeLa cells, treated with paclitaxel (1uM, 24hours), using Lamin A (Phospho-Ser22) antibody.