• Catalog Number:

  E1A8453A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q15942

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  ZYX; Zyxin 2

• Specificity Sensitivity:

  Zyxin (Phospho-Ser142) antibody detects endogenous levels of Zyxin only when phosphorylated at serine 142.

• References:

  Macalma T., J. Biol. Chem. 271:31470-31478(1996). Zumbrunn J., Eur. J. Biochem. 241:657-663(1996). Hillier L.W., Nature 424:157-164(2003). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser142

Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using Zyxin (Phospho-Ser142) antibody.